I am not positive, but I think the cel is from a 
Warner Brothers cartoon feauring Daffy Duck. It's 
a spoof on Dick Tracey. Daffy gets led into a 
room with a bunch of weird villians, and this is 
one of them. I can't remember his name, but he's 
sitting on a train as he tries to ram Daffy.

At least, I think that's the episode.   =)